When the player loses, the song becomes distorted, and one life is lost (represented by rolls of toilet paper). When the player loses all of their lives, the toilet closes.
If the player loses, the song is distorted and part of the katana (the life bar) is lost.
If the player loses, also, a small Wario will go into a "Recycle Bin", if the player loses all of their lives, the words "GAME OVER" appear, before cutting to the score screen.